Gray boxes indicate sense and antisense DNA templates. Blue letters show the 3ʹ-terminal bases in allele-specific primers, whereas red letters show C↔G transversion polymorphisms in sense and antisense DNA templates. Dotted boxes indicate base pairings at SNP sites between allele-specific primers and sense or antisense DNA templates.
In the DNA duplex of a gene, there are weak bonds between opposing pairs of adenine (A) and thymine (T) bases and between guanine (G) and cytosine (C) bases. The matched sense and antisense strands of DNA complement each oth­ er. During the transcription of DNA into RNA, the antisense DNA strand acts as a template for assembling a complementa­
Normally one strand of siRNA (sense or antisense) were needed for RISC to activate the effector molecules (Mostly, argonaut proteins) to carry out gene silencing or target mRNA degradation.
Antisense oligonucleotides are small fragments of DNA that can form complementary pairs with a target mRNA. Intracellularly, the oligo and mRNA hybrid is cleaved by an enzyme, ribonuclease H (RNAse H). RNAse H-mediated cleavage of the RNA strand from the RNA-DNA duplex results in the mRNA being unable to be translated to protein [214]. In our study, the 2′ FU, 2′ FC modification was introduced in the sense or/and antisense strands of siRNA1 duplexes. For s-2′ F, as-2′ F or as/s-2′ F, the 10 th and 11 th nucleotides of the sense or antisense strands, or both strands were modified with 2′-fluoro, respectively . According to a reported mechanism in which the cleavage . 132 158 310 365 318 381 435 1

dna sense vs antisense